Revista de química alimentaria experimental

Functional Variation of Soluble Polyphenols in Oak Apple Gall and Pomegranate Peels and their Inhibition Activity in Leukaemia K562 Cells

Abstract

Chrow Khurshid*,Osamah Ahmed

The possibility of finding anti-cancer drugs has generated interest in natural products. Several studies have in vitro observed potent anti-cancer properties of pomegranate juice against various cancers including leukaemia. Although a few studies have described the bioactivities of hydrolysable tannins extracted from pomegranate juices, limited attention has been paid to other tannins extracted from different parts of a plant. Recently, polyphenols, which are found in plants, have become the most studied phytochemicals owing to their significant chemical properties and biological activities. Tannin is an astringent plant polyphenolic compound and has been observed to have anti-oxidant and anti-cancer properties. This study uses a novel approach to study variation in the structure of hydrolysable tannins from two different types of plants and their inhibition activity in leukaemia K562 cells. We examined data showing anti-proliferation activity of hydrolysable tannins extracted from pomegranate peels within two concentrations of 1 mg/mL and 100 μg/mL. Hydrolysable tannin extracted from oak apple gall induces different effects than that extracted from pomegranate tannin. At the highest concentration of 100 μg/mL, the tannin from oak apple gall did not have any noticeable effects on cells, whereas at the highest concentration of 1 mg/mL, cell cycle arrest seemed to have occurred after 72 h of treatment. This most likely led to senescence caused by overstimulation of the cells by specific polyphenols compounds in oak apple gall, which affected the cells either directly or indirectly by changing the culture’s environment.