Revista de microbiología industrial y alimentaria

Sub-Clinical Mastitis Detection in Can Milk and Isolation of Causative Organisms

Abstract

Thapa Sangita

Milk is a nutritional substance consumed as fluid with minimal processing which is also a raw material for wide variety of products. All the nutritional components that make milk and milk products an important part of the human diet also support the growth of pathogenic organisms. Mastitis is the most costly disease of dairy animals as it reduces milk yield and results in partial or complete damage to udder tissues and decreases the productive life span of animal. This study was conducted to detect the presence of mastitis milk and identify the causative organism of mastitis. A total of 188 samples were analysed. Out of 188 samples, SLST test (modified California Mastitis Test) reaction score were distributed as; negative 44.68% (n=84), score 1+ 22.87% (n=43), score 2+ 17.02% (n=32) and score 3+ 15.42% (n=29). The relationships between SLST test and Somatic Cell Counts was found to be statistically significant. Also, SLST test and salt test showed statistically significant relationship (P<0.05). The causative organisms were isolated from SLST positive milk samples (n=44). Staphylococcus aurous was predominant followed by Coagulase negative Staphylococcus and then Streptococcus agalactiae.