Revista de microbiología industrial y alimentaria

Optimization of Cyclodextrin Glycosyltransferase Production from Sorghum

Abstract

Bernardi NZ, Blanco KC, Monti R and Contiero J

A central composite experimental design was used to evaluate optimal conditions of the medium component, pH and temperature for cyclodextrin glycosyltransferase production from sorghum by Bacillus circulans (American Type Culture Collection). Sorghum concentration, initial pH and temperature were optimized using the response surface method. Maximal CGTase production of 216.94 U.mL-1 was obtained after 48 hours of fermentation when the concentrations of the medium were 0.16% sifted sorghum, 0.5% triptone, 0.5% yeast extract, 1% sodium carbonate, 0.1% K2HPO4, 0.02% MgSO4•7 H2O, initial pH of 8.0 and temperature of 35°C under rotational shaking. Further studies aimed at increasing the fermentation scale using optimal conditions for the high enzymatic production and high volumetric productivity are needed to improve the fermentation process described in the present study.