Medicina molecular y genética

Circular Polymerase Extension Reaction-based Rapid Production of Recombinant Flaviviruses

Abstract

Emeka Nwosu

The Circular Polymerase Extension Reaction (CPER) is a cutting-edge molecular technique that enables rapid and efficient production of recombinant Flaviviruses. This method harnesses the power of circular DNA templates and DNA polymerase to amplify and assemble viral genomes in a single reaction, offering advantages in terms of speed, scalability, and flexibility compared to traditional cloning methods. In this comprehensive review, we delve into the principles, applications, and recent advancements of CPER in the context of Flavivirus research. We discuss its potential impact on vaccine development, pathogenesis studies, and antiviral drug discovery. Furthermore, we explore the challenges and future directions of CPER-based approaches, highlighting its significance in advancing our understanding and combatting Flavivirus infections.