Revista de farmacognosia y productos naturales

Fruit Germination and Tissue Culture Modified MS Media Formula with Estimation of Embelin content in suspension culture by Spectrophotometric Estimation on various tabulated samples of Embelia ribes BURM.F.

Abstract

Syed Asadulla

Germination of Fruits from Rajasthan (Ajmer) variety is Authenticated with accession Collection No. 55181e followed by Gaseous and
surface sterilization were impinged in purified water over night and fruits mixed with concentrated Sulphuric acid for one hour and continue
with 1% HCL moistened for one week then washed with distilled water three times and drained, and excess of acid neutralised with 10%
calcium carbonate Washed with distilled water and confirmed that drained water is Neutral pH. Twenty fruits were transfer to a Sterilized
petri dishes of four inch dia were opened cautiously in a laminar flow, a circular germinating paper inserted and 10 ml of Half concentration
of MS liquid media with 1000 μ Gibbrellic acid per liter of media from (20 ml) was transferred to petri dish and 20 fruits were dispersed on
circular special germinating filter paper and petri dish is sealed with wax elastic paper and transferred to storage racks in light and day room
(12 hours change) at 25 degrees temperature,