Biología molecular: acceso abierto

Conservation of Properties of Outer Membranes Protein Across Host Genera of Pasteurella multocida Suggests Common Mechanism of Action

Abstract

Monendra Grover

Pasteurella multocida is a non-motile coccobacillus pathogenic Gram-negative bacterium and belongs to Pasteurellaceae family. Pasteurella multocida causes diseases in economically important animals and birds in developing and developed countries. Haemorrhagic septicaemia in cattle and buffaloes and other diseases like fowl cholera (turkey, chicken, and duck), Septicaemic pasteurellosis (sheep, pig, and goat) and Snuffles (rabbit) are caused by Pasteurella multocida. In this analysis we have taken three outer membrane proteins (vacJ, ompW and skp) from P. multocida which are involved in infectious diseases of animals and birds. The literature shows that all three outer membrane proteins are infectious in nature. This is supported by multiple sequence alignment and analysis of physicochemical properties of protein sequences encoded by vacJ, ompW and skp outer membrane protein families. The studied proteins are similar with respect to number of positively and negatively charged amino acids, molecular weight, Theoretical pI, instability index and grand average of hydropathicity (GRAVY) in the proteins from P. multocida strains infecting different genera. The domains, transmembrane helices, twin arginine signal peptides and ß-barrels are also broadly similar. This suggests common mechanism of action of these proteins across host genera.