Revista de citología e histología

New Possible Approach in Treatment of Experimental Induced Vaginal Atrophy by Bone Marrow-Derived Mesenchymal Stem Cells in Female Albino Rats (Histological Immunohistochemical and Biochemical Study)

Abstract

Eman Mohamed Faruk, Sherifa Abd El-Salam, Samia Mahmoud Manawy and Naglaa Yehia Nafie

Women after menopause have a lot of complains that negatively distress their life. In the modern years, a lot of conduction methods have been presented to relief undesirable symptoms. Bone marrow mesenchymal stem cells (BM-MSCs) can be recently used as a new therapeutic method treatment of many diseases and avoidance the hormonal therapy symptoms and complications sides effects that happened after menopause.

Aim: The aim of this research is to show a new approach in modification of the structure of vaginal mucosal atrophy by uses of (BM-MSCs) in induced ovariectomized rats.

Methods and results: Fifty female albino rats were used and divided randomly into five groups: control group, ovariectomized group, ovariectomized group plus estrogen (20 ug/kg/day for 4 weeks), ovariectomized with BMMSCs (107 MSCs/rat intravenously) and ovariectomized rats with stem cells/BM-MSCs Intra-vaginal group, the expression of genes for GAPDH, iNOS and TGF-β were done and vaginal biopsies were taken for histological and immunohistochemical studies. In ovariectomized group there was inflammation, ulceration and re-epithelialization with irregularity in collagen fibers, decreased estrogen receptors expression and expression of TGF-β, GAPDH and iNOS were very high. While the rate of healing of epithelium was increased with increased the vasculatures of vaginal mucosa and the estrogen receptors expression was high with decreased expression of GAPDH, iNOS and TGF-β in ovariectomized rats that treated with intra-vaginal group BM-MSCs.

Conclusions: Using the BM-MSCs could be used intravaginal safely in case of vaginal atrophy as they modify the structure of vaginal mucosa than estrogen hormone therapy alone.