Hassan GM and Mazher KHM
Fat released from chicken during grilling process, as waste, is used by some low income people in Egypt in cooking as dietary fat because it is a cheap fat source compared to other fats. The present study aimed to elucidate the genotoxic and histopatholgic effects of grilled chicken fat on the liver, kidney and lymphocytes of male albino rats. Fifteen two months old male rats were divided to three groups, the first group was fed on control diet, the second and third groups were fed diets containing wasted fat at substitution levels of 50 and 100% from the fat of diet, respectively. The animals were fed ad-libtium for eight weeks. The single cell gel electrophoresis (Comet) assay is a simple and effective method for detecting DNA damage in control and treated rat cells, and results showed significant increase in tailed nuclei (DNA damage), tail moment , % DNA in the tail and tail length in liver , kidney and lymphocytes of treated groups compared to the control.In addition, both liver and kidney of treated rats showed a marked degenerative changes congestion of blood vessels in addition to loss of protein, polysaccharides and DNA content.
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