Daniela Fantini Vale, Renato Martins da Silva, Raquel Ribeiro de Aguiar, Guilherme Alexandre Monteiro, Fernanda Antunes, Renato Abdala Karam Kalil, Carlos Jorge Logullo de Olveira and André Lacerda de Abreu Oliveira
Ischemia is responsible for several heart injuries, leading to functional disorders and higher mortality in animals. This process is a condition of blood circulatory arrest, leading to hypoxia and an anaerobic glycolysis. In this case, glycogen is fundamental to maintain energy homeostasis, through glycogen synthase kinase 3 (GSK3) regulations. This enzyme is usually involved in cardio protection, as well as several other biological processes. To study glycogen synthase kinase 3β (GSK3β), analyzing the involvement of this enzyme on cardiac system protection to understand its role in energetic metabolism during ischemia and reperfusion. Using the inflow occlusion (IO) application, the circulatory blood to the heart was blocked in adult New Zealand white rabbits. Parameters such hemogasometry as lactate levels were evaluated during the transoperatory period, using CG4+test strips (i-STAT® System). GSK3β transcription and activity analysis was performed by real time qRT-PCR and western blotting respectively, and glycogen quantification was determined enzymatically.GSK3β transcription increased during ischemia, followed by a decrease in glycogen content, suggesting that the consumption of this substrate during ischemia is mediated by GSK3β. Lactate level is highest in ischemia, and the pH value. decreased during the same period. The results suggest the importance of GSK3β in the heart metabolic adaptations after ischemia and reperfusion injuries, sustaining glucose anaerobic metabolism through glycogen reserves modulation. The results show that the transcription of GSK3β correlated with cardiac metabolic adaptations after ischemia and reperfusion injuries, sustaining glucose anaerobic metabolism.
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