Química medicinal

Spectroscopic Investigation on the Interaction of Pb(II) with Keyhole Limpet Hemocyanin

Abstract

Yulan Fan, Huifang Chen, Guidi Zeng, Jingyi Liu, Jun Xue, Yongquan Wu and Xun Li

The interaction between Pb2+ and Keyhole Limpet Hemocyanin (KLH) was investigated by fluorescence, Ultraviolet-visible (UV-Vis) absorption and Circular Dichroism (CD) spectroscopy. The experimental results showed that Pb2+ could quench the intrinsic fluorescence of KLH following static and dynamic quenching process. The number of binding sites n was approximately equal to 1. ΔH<0 and ΔS<0 indicated that a Pb-KLH complex was formed. Furthermore, the data of synchronous fluorescence, UV-Vis and CD spectra suggested that Pb2+ changed the micro-environment and conformation of KLH.