Revista de SIDA e investigación clínica

Nanomagnet-Based Detoxifying Machine: An Alternative/Complementary Approach in HIV therapy

Abstract

Khaled Seidi, Ali Eatemadi, Behzad Mansoori, Rana Jahanban-Esfahlan and Davoud Farajzadeh

Although the use of highly active antiretroviral therapy (HAART) hampers HIV-1 replication, it is not yet a cure. The main obstacle to HAART-mediated HIV eradication is the latent reservoir of virally infected cells, which is the source of fast viral rebound in patients that stopped therapy. Patients must therefore have lifelong HAART, which is expensive and in long term the many side effects undermine successful treatment. Attempts to eradicate latent reservoir have focused on reactivating latent proviruses. However, killing the infected cells after virus reactivation, which is pivotal for elimination of the reservoir, has not been addressed. In this paper, we proposed a novel complementary approach, which we have called the “Nanomagnet-Based Detoxifying Machine (NBDM)” and, is able to eliminate the virus load in the bloodstream of the patients. In this regard, our proposed nanomagnets (Fe3O4-PLGA-PEG-gp120 Ab\aptamer\ MIP) will be able to bind to any gp120 markers in the blood circulation. The nanomagnets will trap the virus particles in a dialysis column during the hemodialysis and under a strong electromagnetic field. Therefore, each nanomagnets will serve as a hook to capture millions of virus particles out of the patient’s body. In the present research, we suggested the application of the Molecularly Imprinted Polymers (MIPs), because they could simply be synthesized through selfassembling approaches without any need for highly expensive Ab/aptamers. The synthesized MIPs have the potential to mimic the behavior of an antibody/aptamer raised against one of the main antigenic determinants of the virus, such as gp120. NBDM provides a simple but very beneficial system to detoxify the blood from both the viruses and nanoparticles. In conclusion, we think that it could be used as complementary therapy beside the HAART to improve the CD4+ count and disease prognosis without any concern for side effects. This is a simple, cost effectiveness, easy to handle, convenient for the patients, non-invasive, and effective approach that could be extended for a large spectrum of human diseases other than HIV.