Revista de Ciencias Biomédicas y Farmacéuticas

Isolation and Molecular Characterization Of Aspergillus fumigatus From Hunting Dogs With Special Emphasis To Age And Gender As A Risk Factors In Diyala Province â??Iraq

Abstract

Ali Ibrahim Ali Al-Ezzy , Sarah Jasim Abdulameer

Aim: The current study aims to isolation and molecular characterization of A. fumigatus from hunting dogs and evaluation of relationship between A. fumigatus infection and possible risk factors mainly age and gender Methods: Ninety nine swabs from mouth, nose and ear of hunting dogs with respiratory signs were cultured on Sabouraud Dextrose Agar. A. fumigatus was identified according to morphology and PCR technique Results: A.fumigatus was isolated frequently from mouth of hunting dogs at the age group (5.4 -6.2) years followed by (0.6-1.5) years and (2.5-3.3)years,(6.06%) . A.fumigatus was isolated equally from nose of hunting dogs at the age group (0.6-1.5)years ,(1.6-2.4 ) years , ( 2.5-3.3 )years,( 4.3-5.3) years and (5.4-6.2) years, (3.03%). A.fumigatus was isolated frequently from ear of hunting dogs at the age group (5.4-6.2)years;(9.09%). No significant difference was reported between age groups of hunting dogs infected with A.fumigatus . Significant correlation was reported between older age group of hunting dogs and A.fumigatus infection for ear. Current study revealed that (63.64%) of hunting dogs were males while female represent (36.36%).Both genders were equally infected with A.fumigatus isolated from mouth and nose, (15.15%) and (6.06%) respectively .Females were infected more than males with A.fumigatus isolated from ear,(6.06%) Neither significant difference nor correlation were reported between genders of hunting dogs infected with A.fumigatus. Males appear to be at risk of getting A.fumigatus infection at( 2.286) time than females. Conclusions: A. fumigatus infection represent serious problem for hunting dogs . Mouth, nose and ear respectively exposed.