Química medicinal

Evaluation of Antimicrobial Activity of Various Chemicals on Isolated Chicken and Mutton Spoilage Microorganisms

Abstract

Bilal Ahmad Mir, Amin Mir, Dhyal Singh and Zainab Rao

The study was carried out on the isolation of various micro-organisms, in which various samples of chicken and mutton were collected from different locations of the Dehradun. The process of slaughtering of the chicken and mutton as per the survey was of two types- viz, Jatka and Halal. The isolated micro-organisms from the chicken samples of Jatka type include- Vibrio, Enterobacter species, Clostridium, E. coli, Shigella, whereas the micro-organisms isolated from the chicken samples of Halal type include only Enterobacter species, Clostridium and E. coli. The micro-organisms isolated from the mutton sample of Jatka type include, Shigella, E. coli, Vibrio, Staphylococcus, and the micro-organisms isolated from the mutton samples of Halal type include only E. coli, Vibrio and Staphylococcus. The micro-organisms have been isolated and identified by various biochemical tests. The isolated micro-organisms have been then testified for antimicrobial susceptibility test against various chemical substances which include, Gentamicin, Naphthazarin, Azithromycin, Citric Acid, Amphotericin-B, Clindomycin, Cefixime, Norfloxacin, Streptomycin, Ampicilin. Among the various used chemical substances, Gentamicin have been found effective in inhabiting the growth of all the isolated micro-organisms, followed by Norfloxacin, Streptomycin, Ampicilin, Azithromycin, Citric Acid. Least antimicrobial activity was observed for Naphthazarin, Amphotericin-B, Clindomycin, and Cefixime. Among the isolated micro-organisms shigella species was found to be less proned against the applied anti-microbial drugs, and the most susceptible micro-organism among the isolated micro-organisms includes E. coli. Also from the collected chicken and mutton samples the samples of Halal type have been to be less effective against the growth of micro-organisms as compared to the Jatka type samples, which could be due to different procedure of slaughtering technique.