Química medicinal

Cytoprotective Properties of Antioxidant Protein from Curry leaves (Murraya koenigii L.) against Oxidative Stress Induced Damage in Human Erythrocytes

Abstract

Mylarappa B Ningappa, Dinesha Ramadas, Dinesha Nanjegowda, Kiruthika Balasubramanian, Khusdeep Chahal, Sachin Patil and Leela Srinivas

Oxidative stress induced by reactive oxygen species (ROS) cause lipid peroxidation at the human erythrocyte membrane with subsequent alterations in the ATPases function. In this study, potential cytoprotective activities of previously reported ”Antioxidant Protein from Curry leaves (APC)’’ in erythrocytes against reactive oxygen (ROS) species generated by pro-oxidants in vitro. The APC prevented red blood cell lysis induced by pro-oxidants; Fe: As (2:20 μmole), Hydrogen peroxide (0.2 mM), and tertiary butyl hydroperoxide (1 mM) upto 97.5, 82.5 and 63%, respectively. Further, APC prevented Fe:As induced K+ leakage in red blood cells up to 95%. The inhibition offered by APC on K+ leakage was comparable to inhibition offered by quinine sulphate, a known K+ channel blocker. Interestingly, the APC at dose dependently restored Na+K+ ATPase and Ca2+Mg2+ ATPase activities of erythrocyte membrane when altered by ROS. The restoration of ATPase activity by APC was two times more than standard antioxidants BHA and α-tocopherol. In conclusion, Curry leaves protein antioxidant is an effective antioxidant in preventing membrane damage and associated functions mediated by reactive oxygen species. It can be further developed as an effective bioprotective antioxidant agent to cellular components.